Advanced Quantitative Fluorescence Microscopy to Probe the Molecular Dynamics of Viral Entry, Science Lab

Viral entry into the host cell requires the coordination of many cellular and viral proteins in a precise order. Modern microscopy techniques are now allowing researchers to investigate these interactions with higher spatiotemporal resolution than ever before. Here we present two examples from the field of HIV research that make use of an innovative quantitative imaging approach as well as cutting edge fluorescence lifetime-based confocal microscopy methods to gain novel insights into how HIV fuses to cell membranes and enters the cell.

Advanced Quantitative Fluorescence Microscopy to Probe the Molecular Dynamics of Viral Entry, Science Lab

Optimized production and fluorescent labeling of SARS-CoV-2 virus-like particles

Probing the Allosteric Inhibition Mechanism of a Spike Protein Using Molecular Dynamics Simulations and Active Compound Identifications

Processes, Free Full-Text

Molecular and Immunological Diagnostic Tests of COVID-19: Current Status and Challenges - ScienceDirect

HIV-1 binding and fusion events observed using confocal fluorescence

Beyond mass spectrometry, the next step in proteomics

Macrophage Sult2b1 promotes pathological neovascularization in age-related macular degeneration

2021 Top 10 Innovations The Scientist Magazine®

Viruses, Free Full-Text

Microbial pathogenesis revealed by intravital microscopy: pros, cons and cautions - Stolp - 2016 - FEBS Letters - Wiley Online Library

Biomolecular condensates modulate membrane lipid packing and hydration

Primate-conserved carbonic anhydrase IV and murine-restricted LY6C1 enable blood-brain barrier crossing by engineered viral vectors

Dynamic Oligomerization of Integrase Orchestrates HIV Nuclear Entry