Pathogens, Free Full-Text
Por um escritor misterioso
Last updated 09 julho 2024
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In Brazil, blood donation is regulated by the Brazilian Ministry of Health, and all States follow the same protocol for clinical and laboratory screening. Brazil is an endemic country for Chagas disease (CD), caused by Trypanosoma cruzi, and for leishmaniasis, caused by a species of Leishmania spp. Screening for leishmaniosis is not routinely performed by blood banks. Given the antigenic similarity between T. cruzi and Leishmania spp., cross-reactions in serological tests can occur, and inconclusive results for CD have been found. The objective of this study was to apply molecular techniques, e.g., nPCR, PCR, and qPCR, to clarify cases of blood donation candidates with non-negative serology for CD and to analyze the difference between the melting temperature during real-time PCR using SYBR Green. Thirty-seven cases that showed non-negative results for CD using chemiluminescent microparticle immunoassay (CMIA) tests from blood banks in Campo Grande, MS, and Campinas, SP, were analyzed. In the serum samples, 35 samples were evaluated by ELISA, and 24.3% (9/35) showed positive results for CD. nPCR was able to detect 12 positive results in 35 samples (34.28%). qPCR for T. cruzi was quantifiable in the samples that showed a value ≥0.002 par eq/mL (parasite equivalents per milliliter), and in 35 samples, 11 (31.42%) were positive. Of all evaluated samples using the described tests (CMIA, ELISA, nPCR, and qPCR), 18 (48.6%) were positive for CD. For MCA by qPCR, the melting temperature was 82.06 °C ± 0.46 for T. cruzi and 81.9 °C ± 0.24 for Leishmania infantum. The Mann–Whitney test showed a significant value of p < 0.0001. However, the differentiation between T. cruzi and L. infantum could not be considered due to temperature overlap. For leishmaniasis, of the 35 samples with non-negative serology for CD tested by the indirect fluorescent antibody test (IFAT), only one sample (2.85%) was positive (1:80). The PCR for Leishmania spp. was performed on 36 blood samples from donation candidates, and all were negative. qPCR for L. infantum showed 37 negative results for the 37 analyzed samples. The data presented here show the importance of performing two different tests in CD screening at blood banks. Molecular tests should be used for confirmation, thereby improving the blood donation system.
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Pathogens and Immunity
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A culture-free biphasic approach for sensitive and rapid detection of pathogens in dried whole-blood matrix
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Pathogens, Free Full-Text
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Pathogens Packet - Bacteria Viruses Protozoa Fungi Parasites and Prions - Homeschool Den
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Read et al. warned us 2015 using chickens (Marek's disease) that using an imperfect 'leaky' vaccine can enhance transmission of highly virulent pathogens; sub-optimal non-sterilizing non-neutralizing
![Pathogens, Free Full-Text](https://iiif.elifesciences.org/lax/68874%2Felife-68874-fig2-v1.tif/full/1500,/0/default.jpg)
Evolution of pathogen tolerance and emerging infections: A missing experimental paradigm
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Pathogen Definition and Examples - Biology Online Dictionary
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Study shows diverse gut bacteria communities protect against harmful pathogens by nutrient blocking
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Disarming the pathogen Nature Chemical Biology
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PDF) Detection and characterization of zoonotic pathogens of free-ranging non-human primates from Zambia.
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