2509444 - safe, edit, edited screencap, screencap, constance, twilight sparkle, bird, pony, unicorn, g4, lesson zero, amogus, among us, crazy smile, faic, female, grin, insanity, jerma985, mare, meme, nest, ponified meme, shitposting

1967389 - safe, screencap, twilight sparkle, alicorn, pony, friendship university, g4, cropped, determined, female, narrowed eyes, serious, twilight sparkle (alicorn), twilight sparkle is not amused, unamused - Derpibooru

1595446 - safe, edit, edited screencap, screencap, spike, twilight sparkle, dragon, pony, unic…

2194860 - safe, screencap, fluttershy, twilight sparkle, alicorn, pony, g4.5, my little pony: pony life, official, animated, cropped, exuberant airdancer fluttershy, female, gif, looking at you, mare, noodle arms, twilight sparkle (alicorn) - Derpibooru

2424944 - safe, screencap, twilight sparkle, pony, unicorn, g4, the crystal empire, butt, female, mare, plot, solo, twibutt, unicorn twilight - Derpibooru

930547 - safe, derpibooru import, screencap, twilight sparkle, pony, unicorn, lesson zero, butt, faic, female, looking back, mare, plot, twibutt, unicorn twilight - Twibooru

1516831 - safe, derpibooru import, edit, edited screencap, screencap, rainbow dash, pony, cursed image, faic, image, jpeg, looking at you, mirrored, not salmon, smiling, smug, smugdash, solo, unitinu, wat, what has science

2178046 - safe, screencap, spike, twilight sparkle, dragon, pony, unicorn, g4, green isn't your color, animated, bed, biting, …

2001255 - safe, edit, edited screencap, screencap, applejack, dance magic, equestria girls…

2194955 - safe, screencap, g4.5, my little pony: pony life, logo, my little pony logo, no pony, photo, simple b…

2268926 - safe, screencap, character:twilight sparkle, character:twilight sparkle (alicorn), species:alicorn, species:pony, episode:once upon a zeppelin, g4, my little pony: friendship is magic, cropped, solo, twilight is not amused, unamused - Manebooru

2961358 - safe, artist:l4zy_4le, screencap, twilight sparkle, alicorn, human, pony, a trivial pursuit, g4, angry, clothes, faic, female, happy, humanized, long sleeves, open mouth, open smile, pinpoint eyes, scene interpretation, screencap reference

2214504 - safe, screencap, mean twilight sparkle, pony, the mean 6, cropped, evil grin, female…

Twilight Not Amused by illumnious on DeviantArt